The Use of Transgenic Resistant Plums in Transgrafting Fails to Confer Plum Pox Virus Resistance in Prunus

In order to sustain productivity, growers are implementing fruit trees provide plum pox virus (PPV) resistant varieties. Unfortunately, classical breeding approaches have failed develop RNA interference (RNAi) silencing, as an alternate strategy, has been shown be efficient approach combat PPV disease (sharka). resistance based on RNAi demonstrated in natural conditions for over 10 years. Experiments using graft inoculation high-containment greenhouses show that silencing protects against multiple dormancy cycles. While the spreads throughout vascular tissues of susceptible hosts, movement is undetectable clones. However, this work, when PPV-susceptible cultivars were grafted onto transgenic rootstocks, small interfering (siRNA) stopped systemic spread into shoots but did not appear prevent infection scions. Thus, we do convincing evidence siRNA transfer nor provision scion. Notably, <em>Macrolophus</em> insects experimentally fed plums could suck from leaves attempt control through transgrafting a new technology (NBT) cannot confirmed, these studies insight key regulatory interactions associated with accumulation and its environmentally safe use perennial plants.